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Human PCYT1A Knockdown Cell Line (Wb-Validated) #C63313

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RT-qPCR analysis. HeLa cells were infected with PCYT1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PCYT1A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PCYT1A and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PCYT1A
  • 货号：
    C63313
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human PCYT1A Knockdown Cell Line (Wb-Validated)
Aliases PCYT1A; Phosphate Cytidylyltransferase 1A, Choline;
CTPCT; CCTalpha; PCYT1; CT; Phosphate Cytidylyltransferase 1, Choline, Alpha; CTP:Phosphocholine Cytidylyltransferase-Alpha; Choline-Phosphate Cytidylyltransferase Alpha; CTP:Phosphocholine Cytidylyltransferase A; Choline-Phosphate Cytidylyltransferase A; Phosphorylcholine Transferase Alpha;
Phosphorylcholine Transferase A; EC 2.7.7.15 4; CCT-Alpha; CCT A; CT A;
Phosphate Cytidylyltransferase 1, Choline, Alpha Isoform; EC 2.7.7; SMDCRD; CCTA; CGL5;CTA
Background

Gene Name: PCYT1A

NCBI Gene Entry: 5130
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PCYT1A Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PCYT1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PCYT1A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PCYT1A and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    PCYT1A
  • 货号：
    C63313
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PCYT1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PCYT1A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PCYT1A and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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