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Human IFIH1 Knockdown Cell Line (Wb-Validated) #C61179

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RT-qPCR analysis. HT-1080 cells were infected with IFIH1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. IFIH1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against IFIH1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    IFIH1
  • 货号:
    C61179
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human IFIH1 Knockdown Cell Line (Wb-Validated)
Aliases IFIH1; Interferon Induced With Helicase C Domain 1; MDA-5; MDA5; Helicard; IDDM19; Interferon-Induced Helicase C Domain-Containing Protein 1; Clinically Amyopathic Dermatomyositis Autoantigen 140 KDa; Melanoma Differentiation-Associated Protein 5; Melanoma Differentiation-Associated Gene 5; RNA Helicase-DEAD Box Protein 116; Murabutide Down-Regulated Protein; Helicase With 2 CARD Domains; RIG-I-Like Receptor; CADM-140 Autoantigen; RLR-2; Hlcd; Interferon-Induced With Helicase C Domain Protein 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide; EC 3.6.4.13; SGMRT1; IMD95; RH116; AGS7; HLCD
Background

Gene Name: IFIH1

NCBI Gene Entry: 64135

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human IFIH1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with IFIH1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. IFIH1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against IFIH1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HT-1080 cells were infected with IFIH1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. IFIH1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against IFIH1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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