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Human HSPA9 Knockdown Cell Line (Wb-Validated) #C61525

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RT-qPCR analysis. HeLa cells were infected with HSPA9-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. HSPA9 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPA9 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    HSPA9
  • 货号:
    C61525
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human HSPA9 Knockdown Cell Line (Wb-Validated)
Aliases HSPA9; Heat Shock Protein Family A (Hsp70) Member 9; GRP75; PBP74; 75 KDa Glucose-Regulated Protein; Stress-70 Protein, Mitochondrial; HSPA9B; Heat Shock 70kDa Protein 9 (Mortalin); Peptide-Binding Protein 74; Mortalin2; Mortalin; MTHSP75; GRP-75; MOT; Epididymis Secretory Sperm Binding Protein Li 124m; Heat Shock 70kDa Protein 9B (Mortalin-2); Catecholamine-Regulated Protein 40; Heat Shock 70 KDa Protein 9; Heat Shock 70kD Protein 9B; Mortalin, Perinuclear; P66-Mortalin; HEL-S-124m; Mortalin-2; Mt-HSP70; Mthsp75; SIDBA4; Mot-2; CRP40; EVPLS; MOT-2; MOT2; SAAN; CSA
Background

Gene Name: HSPA9

NCBI Gene Entry: 3313

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human HSPA9 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with HSPA9-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. HSPA9 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPA9 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with HSPA9-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. HSPA9 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPA9 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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