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Human UCHL1 Knockdown Cell Line (Wb-Validated) #C61191

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RT-qPCR analysis. HAP1 cells were infected with UCHL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. UCHL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HAP1 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against UCHL1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    UCHL1
  • 货号：
    C61191
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human UCHL1 Knockdown Cell Line (Wb-Validated)
Aliases UCHL1; Ubiquitin C-Terminal Hydrolase L1; PGP9.5; UCHL-1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; UCH-L1; PARK5; Epididymis Secretory Protein Li 53; Epididymis Luminal Protein 117; EC 3.4.19.12; HEL-S-53; HEL-117; SPG79A; NDGOA; PGP95; SPG79
Background

Gene Name: UCHL1

NCBI Gene Entry: 7345
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human UCHL1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HAP1 cells were infected with UCHL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. UCHL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HAP1 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against UCHL1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    UCHL1
  • 货号：
    C61191
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HAP1 cells were infected with UCHL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. UCHL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HAP1 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against UCHL1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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