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Human STUB1 Knockdown Cell Line (Wb-Validated) #C61243

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RT-qPCR analysis. HeLa cells were infected with STUB1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. STUB1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61243, 1:5,000) against STUB1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    STUB1
  • 货号:
    C61243
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human STUB1 Knockdown Cell Line (Wb-Validated)
Aliases STUB1; STIP1 Homology And U-Box Containing Protein 1; CHIP; SDCCAG7; HSPABP2; NY-CO-7; UBOX1; Carboxy Terminus Of Hsp70-Interacting Protein ; RING-Type E3 Ubiquitin; Transferase CHIP; E3 Ubiquitin-Protein Ligase CHIP; CLL-Associated Antigen KW-8; Antigen NY-CO-7; STIP1 Homology And U-Box Containing Protein 1, E3 Ubiquitin Protein Ligase; Heat Shock Protein A Binding Protein 2 (C-Terminal); STIP1 Homology And U Box-Containing Protein 1; Serologically Defined Colon Cancer Antigen 7; EC 2.3.2.27; SCAR16; SCA48
Background

Gene Name: STUB1

NCBI Gene Entry: 10273

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human STUB1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with STUB1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. STUB1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61243, 1:5,000) against STUB1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
RT-qPCR analysis. HeLa cells were infected with STUB1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. STUB1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61243, 1:5,000) against STUB1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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