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Human CC2D1A Knockdown Cell Line (Wb-Validated) #C61327

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RT-qPCR analysis. HeLa cells were infected with CC2D1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CC2D1A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against CC2D1A and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    CC2D1A
  • 货号：
    C61327
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human CC2D1A Knockdown Cell Line (Wb-Validated)
Aliases CC2D1A; Coiled-Coil And C2 Domain Containing 1A; Five Prime Repressor Element Under Dual; Repression-Binding Protein 1; Akt Kinase-Interacting Protein 1; MRT3; TAPE; Coiled-Coil And C2 Domain-Containing Protein 1A; FRE Under Dual Repression-Binding Protein 1; Putative NF-Kappa-B-Activating Protein 023N; TBK1-Associated Protein In Endolysosomes(多2); Lethal (2) Giant Discs Homolog 2; FLJ20241; Freud-1; Aki-1; Lgd2; Five Repressor Element Under Dual Repression-Binding Protein 1; Mental Retardation, Nonsyndromic, Autosomal Recessive, 3; Putative NFkB Activating Protein; Freud-1/Aki1; LGD2; AKI1(多FREUD-1;AKI-1)
Background

Gene Name: CC2D1A

NCBI Gene Entry: 54862
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human CC2D1A Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with CC2D1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. CC2D1A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against CC2D1A and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    CC2D1A
  • 货号：
    C61327
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with CC2D1A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CC2D1A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against CC2D1A and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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