• 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类

Human CBFB Knockdown Cell Line (Wb-Validated) #C61442

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RT-qPCR analysis. HeLa cells were infected with CBFB-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CBFB protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin α served as a loading control. The blots were incubated with primary antibodies (Cat#61442, 1:5,000) against CBFB and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CBFB
  • 货号:
    C61442
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human CBFB Knockdown Cell Line (Wb-Validated)
Aliases CBFB; Core-Binding Factor Subunit Beta; PEBP2B; SL3/AKV Core-Binding Factor Beta Subunit; SL3-3 Enhancer Factor 1 Subunit Beta; Core-Binding Factor Beta Subunit; PEBP2-Beta; PEA2-Beta; CBF-Beta; Polyomavirus Enhancer Binding Protein 2, Beta Subunit; Polyomavirus Enhancer-Binding Protein 2 Beta Subunit; SL3-3 Enhancer Factor 1 Beta Subunit; Core-Binding Factor, Beta Subunit; CLCD2
Background

Gene Name: CBFB

NCBI Gene Entry: 865

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human CBFB Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with CBFB-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. CBFB protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin α served as a loading control. The blots were incubated with primary antibodies (Cat#61442, 1:5,000) against CBFB and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
RT-qPCR analysis. HeLa cells were infected with CBFB-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CBFB protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin α served as a loading control. The blots were incubated with primary antibodies (Cat#61442, 1:5,000) against CBFB and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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