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Human NDUFS1 Knockdown Cell Line (Wb-Validated) #C65225

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RT-qPCR analysis. HT-1080 cells were infected with NDUFS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NDUFS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NDUFS1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    NDUFS1
  • 货号：
    C65225
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human NDUFS1 Knockdown Cell Line (Wb-Validated)
Aliases NDUFS1; NADH:Ubiquinone Oxidoreductase Core Subunit S1; NADH-Ubiquinone Oxidoreductase 75 KDa Subunit, Mitochondrial; CI-75k; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase); Complex I 75kDa Subunit 2 3NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1 (75kD) (NADH-Coenzyme Q Reductase); Mitochondrial NADH-Ubiquinone Oxidoreductase 75 KDa Subunit; Complex I, Mitochondrial Respiratory Chain, 75-KD Subunit; Complex I-75kD; EC 7.1.1.2; CI-75Kd; PRO1304; CI-75kD; MC1DN5
Background

Gene Name: NDUFS1

NCBI Gene Entry: 4719
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human NDUFS1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with NDUFS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. NDUFS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NDUFS1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    NDUFS1
  • 货号：
    C65225
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HT-1080 cells were infected with NDUFS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NDUFS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NDUFS1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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