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Human LOXL2 Knockdown Cell Line (Wb-Validated) #C61481

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RT-qPCR analysis. HeLa cells were infected with LOXL2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. LOXL2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LOXL2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    LOXL2
  • 货号:
    C61481
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human LOXL2 Knockdown Cell Line (Wb-Validated)
Aliases LOXL2; Lysyl Oxidase Like 2; WS9-14; LOR; Lysyl Oxidase-Related Protein WS9-14; Lysyl Oxidase-Related Protein 2; Lysyl Oxidase-Like Protein 2; Lysyl Oxidase Homolog 2; Lysyl Oxidase-Like 2 Delta E13; Lysyl Oxidase-Like 2 Protein; Lysyl Oxidase Related 2; Lysyl Oxidase-Like 2; EC 1.4.3.13; EC 1.4.3; LOR2
Background

Gene Name: LOXL2

NCBI Gene Entry: 4017

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human LOXL2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with LOXL2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. LOXL2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LOXL2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with LOXL2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. LOXL2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LOXL2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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