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Human MAPK3 Knockdown Cell Line (Wb-Validated) #C61484

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RT-qPCR analysis. HeLa cells were infected with MAPK3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. MAPK3 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61483, 1:5,000) against MAPK3 and  β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    MAPK3
  • 货号:
    C61484
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human MAPK3 Knockdown Cell Line (Wb-Validated)
Aliases MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; P44ERK1; P44MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase; EC 2.7.11; HS44KDAP; HUMKER1A; P44mapk; P44erk1; MAPK 1; MAPK
Background

Gene Name: MAPK3

NCBI Gene Entry: 5595

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human MAPK3 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with MAPK3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. MAPK3 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61483, 1:5,000) against MAPK3 and  β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
RT-qPCR analysis. HeLa cells were infected with MAPK3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. MAPK3 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61483, 1:5,000) against MAPK3 and  β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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