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Human LPCAT1 Knockdown Cell Line (Wb-Validated) #C65211

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RT-qPCR analysis. HeLa cells were infected with LPCAT1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. LPCAT1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LPCAT1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    LPCAT1
  • 货号：
    C65211
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human LPCAT1 Knockdown Cell Line (Wb-Validated)
Aliases LPCAT1; Lysophosphatidylcholine Acyltransferase 1; AGPAT10; AGPAT9; LPLAT8; AYTL2; Acetyl-CoA:Lyso-Platelet-Activating Factor Acetyltransferase; 1-Alkenylglycerophosphocholine O-Acyltransferase; 1-Alkylglycerophosphocholine O-Acetyltransferase; 1-Acylglycerophosphocholine O-Acyltransferase; 1-Acylglycerol-3-Phosphate O-Acyltransferase; Phosphonoformate Immuno-Associated Protein 3; Acetyl-CoA:Lyso-PAF Acetyltransferase; Lysophospholipid Acyltransferase 8; Lyso-PAF Acetyltransferase; LysoPC Acyltransferase 1; Acyltransferase-Like; LPC Acyltransferase 1; LysoPAFAT; FLJ12443; LPCAT-1; PFAAP3; Acyltransferase Like 2; EC 2.3.1.23; EC 2.3.1.51; EC 2.3.1.25; EC 2.3.1.67; Lpcat
Background

Gene Name: LPCAT1

NCBI Gene Entry: 79888
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human LPCAT1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with LPCAT1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. LPCAT1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LPCAT1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    LPCAT1
  • 货号：
    C65211
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with LPCAT1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. LPCAT1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against LPCAT1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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