首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

  • 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类
首页>产品中心>细胞系

Human PFKFB3 Knockdown Cell Line (Wb-Validated) #C61862

一键复制产品信息

返回列表

RT-qPCR analysis. HT-1080 cells were infected with PFKFB3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PFKFB3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61862, 1:5,000) against PFKFB3 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    PFKFB3
  • 货号：
    C61862
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

Citation(0)

Information
Product Name Human PFKFB3 Knockdown Cell Line (Wb-Validated)
Aliases PFKFB3; 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3; 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase Brain/Placenta-Type Isozyme 3; Renal Carcinoma Antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; IPFK-2; Inducible 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase; Fructose-6-Phosphate,2-Kinase/Fructose-2, 6-Bisphosphatase; 6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase; IPFK2; PFK2
Background

Gene Name: PFKFB3

NCBI Gene Entry: 5209
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PFKFB3 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with PFKFB3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PFKFB3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61862, 1:5,000) against PFKFB3 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    PFKFB3
  • 货号：
    C61862
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HT-1080 cells were infected with PFKFB3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PFKFB3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61862, 1:5,000) against PFKFB3 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号