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Human FASN Knockdown Cell Line (Wb-Validated) #C61976

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RT-qPCR analysis. HeLa cells were infected with FASN-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. FASN protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FASN and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    FASN
  • 货号：
    C61976
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human FASN Knockdown Cell Line (Wb-Validated)
Aliases FASN; Fatty Acid Synthase; FAS; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X, Member 1; 3-Hydroxyacyl-[Acyl-Carrier-Protein] DehydRatase; [Acyl-Carrier-Protein] S-Malonyltransferase; [Acyl-Carrier-Protein] S-Acetyltransferase; 3-Oxoacyl-[Acyl-Carrier-Protein] Reductase; 3-Oxoacyl-[Acyl-Carrier-Protein] Synthase; Enoyl-[Acyl-Carrier-Protein] Reductase; Acyl-[Acyl-Carrier-Protein] Hydrolase; Type I Fatty Acid Synthase; EC 2.3.1.85; EC 6.3.3.1; EC 2.3.1; OA-519
Background

Gene Name: FASN

NCBI Gene Entry: 2194
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human FASN Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with FASN-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. FASN protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FASN and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    FASN
  • 货号：
    C61976
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with FASN-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. FASN protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FASN and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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