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Human CD44 Knockdown Cell Line (Wb-Validated) #C61350

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RT-qPCR analysis. HeLa cells were infected with CD44-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CD44 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin α served as a loading control. The blots were incubated with primary antibodies (Cat#61350, 1:5,000) against CD44 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CD44
  • 货号:
    C61350
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human CD44 Knockdown Cell Line (Wb-Validated)
Aliases CD44 Molecule (Indian Blood Group); HUTCH-I; HCELL; CSPG8; MC56; Pgp1; MDU2; MDU3; MIC4; IN; Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Homing Cell Adhesion Molecule; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Phagocyte Glycoprotein 1; Hyaluronate Receptor; In(Lu) Related-P80; Hermes Antigen; CD44 Antigen; Hermes-1; ECMR-III; HUTCH-1; ECM-III; Epican; PGP-1; CD44R; CDw44; H-CAM; LHR; CD44 Antigen (Homing Function And Indian Blood Group System); Homing Function And Indian Blood Group System; Extracellular Matrix Receptor-III; Cell Surface Glycoprotein CD44; Indian Blood Group Antigen; Phagocytic Glycoprotein I; Soluble CD44; CDW44; PGP-I
Background

Gene Name: CD44

NCBI Gene Entry: 960

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human CD44 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with CD44-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. CD44 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin α served as a loading control. The blots were incubated with primary antibodies (Cat#61350, 1:5,000) against CD44 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
RT-qPCR analysis. HeLa cells were infected with CD44-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CD44 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin α served as a loading control. The blots were incubated with primary antibodies (Cat#61350, 1:5,000) against CD44 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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