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Human KDM1B Knockdown Cell Line (Wb-Validated) #C62155

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RT-qPCR analysis. HeLa cells were infected with KDM1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. KDM1B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#62155, 1:5,000) against KDM1B and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    KDM1B
  • 货号:
    C62155
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human KDM1B Knockdown Cell Line (Wb-Validated)
Aliases KDM1B; Lysine Demethylase 1B; LSD2; AOF1; Flavin-Containing Amine Oxidase Domain-Containing Protein 1; Lysine-Specific Histone Demethylase 1B; Lysine-Specific Histone Demethylase; Lysine (K)-Specific Demethylase 1B; DJ298J15.2; BA204B7.3; FLJ34109; FLJ33898; FLJ43328; C6orf193; Amine Oxidase (Flavin Containing) Domain 1; Chromosome 6 Open Reading Frame 193; Amine Oxidase, Flavin Containing 1; EC 1.14.99.66; C6ORF193
Background

Gene Name: KDM1B

NCBI Gene Entry: 221656

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human KDM1B Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with KDM1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. KDM1B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#62155, 1:5,000) against KDM1B and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
RT-qPCR analysis. HeLa cells were infected with KDM1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. KDM1B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#62155, 1:5,000) against KDM1B and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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