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Human NDUFA13 Knockdown Cell Line (Wb-Validated) #C62387

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RT-qPCR analysis. HeLa cells were infected with NDUFA13-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NDUFA13 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against NDUFA13 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    NDUFA13
  • 货号：
    C62387
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human NDUFA13 Knockdown Cell Line (Wb-Validated)
Aliases NADH:Ubiquinone Oxidoreductase Subunit A13; GRIM-19; GRIM19; CGI-39; CDA016; B16.6; Gene Associated With Retinoic And Interferon-Induced Mortality 19 Protein; Gene Associated With Retinoic And IFN-Induced Mortality 19 Protein; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 13; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 13; NADH-Ubiquinone Oxidoreductase B16.6 Subunit; Cell Death Regulatory Protein GRIM-19; Complex I B16.6 Subunit; Complex I-B16.6; CI-B16.6; Cell Death-Regulatory Protein GRIM19; MC1DN28
Background

Gene Name: NDUFA13

NCBI Gene Entry: 51079
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human NDUFA13 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with NDUFA13-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. NDUFA13 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against NDUFA13 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    NDUFA13
  • 货号：
    C62387
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with NDUFA13-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NDUFA13 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against NDUFA13 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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