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Human PSIP1 Knockdown Cell Line (Wb-Validated) #C62438

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RT-qPCR analysis. HeLa cells were infected with PSIP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PSIP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PSIP1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    PSIP1
  • 货号:
    C62438
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human PSIP1 Knockdown Cell Line (Wb-Validated)
Aliases PSIP1; PC4 And SRSF1 Interacting Protein 1; LEDGF; DFS70; Lens Epithelium-Derived Growth Factor; PSIP2; P52; P75; PC4 And SFRS1 Interacting Protein 1; Dense Fine Speckles 70 KDa Protein; PC4 And SFRS1-Interacting Protein; CLL-Associated Antigen KW-7; PC4 And SFRS1 Interacting Protein 2; Transcriptional Coactivator P52/P75; Transcriptional Coactivator P75/P52; DFS 70; PAIP
Background

Gene Name: PSIP1

NCBI Gene Entry: 11168

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PSIP1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PSIP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PSIP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PSIP1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with PSIP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PSIP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PSIP1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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