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Human PRDX6 Knockdown Cell Line (Wb-Validated) #C62453

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RT-qPCR analysis. HeLa cells were infected with PRDX6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PRDX6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRDX6 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    PRDX6
  • 货号:
    C62453
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human PRDX6 Knockdown Cell Line (Wb-Validated)
Aliases Peroxiredoxin 6; AiPLA2; NSGPx; AOP2; KIAA0106; 1-Cys; PRX; P29; Acidic Calcium-Independent Phospholipase A2; Lysophosphatidylcholine Acyltransferase 5; Glutathione-Dependent Peroxiredoxin; Non-Selenium Glutathione Peroxidase; Red Blood Cells Page Spot 12; Lyso-PC Acyltransferase 5; LPC Acyltransferase 5; Antioxidant Protein 2; Liver 2D Page Spot 40; 1-Cys Peroxiredoxin; Peroxiredoxin-6; 24 KDa Protein; 1-Cys PRX; MGC46173; LPCAT-5; Epididymis Secretory Sperm Binding Protein Li 128m; EC 1.11.1.27; EC 1.11.1.15; EC 2.3.1.23; HEL-S-128m; EC 3.1.1.4
Background

Gene Name: PRDX6

NCBI Gene Entry: 9588

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PRDX6 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PRDX6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PRDX6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRDX6 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with PRDX6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PRDX6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRDX6 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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