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Human UBE4B Knockdown Cell Line (Wb-Validated) #C62638

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RT-qPCR analysis. HeLa cells were infected with UBE4B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. UBE4B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against UBE4B and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    UBE4B
  • 货号：
    C62638
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human UBE4B Knockdown Cell Line (Wb-Validated)
Aliases UBE4B; Ubiquitination Factor E4B; UFD2; KIAA0684; UBOX3; E4; Ubiquitination Factor E4B (UFD2 Homolog, Yeast); RING-Type E3 Ubiquitin Transferase E4 B; Ubiquitin Conjugation Factor E4 B; HDNB1; Ubiquitination Factor E4B (Homologous To Yeast UFD2); Homozygously Deleted In Neuroblastoma-1; Homozygously Deleted In Neuroblastoma 1; Ubiquitin-Fusion Degradation Protein 2; Ubiquitin Fusion Degradation Protein 2; Homologous To Yeast UFD2; EC 2.3.2.27; UFD2A
Background

Gene Name: UBE4B

NCBI Gene Entry: 10277
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human UBE4B Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with UBE4B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. UBE4B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against UBE4B and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    UBE4B
  • 货号：
    C62638
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with UBE4B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. UBE4B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against UBE4B and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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