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Human UBR5 Knockdown Cell Line (Wb-Validated) #C62788

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RT-qPCR analysis. HeLa cells were infected with UBR5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. UBR5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against UBR5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    UBR5
  • 货号：
    C62788
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

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Information
Product Name Human UBR5 Knockdown Cell Line (Wb-Validated)
Aliases UBR5; Ubiquitin Protein Ligase E3 Component N-Recognin 5; HYD; EDD; KIAA0896; EDD1; DD5; E3 Ubiquitin-Protein Ligase, HECT Domain-Containing 1; HECT-Type E3 Ubiquitin Transferase UBR5; Hyperplastic Discs Protein Homolog; E3 Ubiquitin-Protein Ligase UBR5; Progestin-Induced Protein; E3 Ubiquitin Protein Ligase, HECT Domain Containing, 1; E3 Identified By Differential Display; EC 2.3.2.26; EC 6.3.2; HHYD
Background

Gene Name: UBR5

NCBI Gene Entry: 51366
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human UBR5 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with UBR5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. UBR5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against UBR5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    UBR5
  • 货号：
    C62788
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with UBR5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. UBR5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against UBR5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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