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Human TACSTD2 Knockdown Cell Line (Wb-Validated) #C65609

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RT-qPCR analysis. HeLa cells were infected with TACSTD2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.TACSTD2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against TACSTD2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    TACSTD2
  • 货号：
    C65609
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human TACSTD2 Knockdown Cell Line (Wb-Validated)
Aliases TACSTD2; Tumor Associated Calcium Signal Transducer 2; GA733-1; TROP2; EGP-1; M1S1; Membrane Component Chromosome 1 Surface Marker 1; Tumor-Associated Calcium Signal Transducer 2; Pancreatic Carcinoma Marker Protein GA733-1; Trophoblast Cell Surface Antigen 2; Cell Surface Glycoprotein Trop-2; Epithelial Glycoprotein-1; 40kD Glycoprotein, Identified By Monoclonal Antibody GA733; Membrane Component, Chromosome 1, Surface Marker 1; Gastrointestinal Tumor-Associated Antigen GA7331; Pancreatic Carcinoma Marker Protein GA7331; Cell Surface Glycoprotein TROP2; GA7331; EGP1; GP50
Background

Gene Name: TACSTD2

NCBI Gene Entry: 4070
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human TACSTD2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with TACSTD2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.TACSTD2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against TACSTD2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    TACSTD2
  • 货号：
    C65609
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with TACSTD2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.TACSTD2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against TACSTD2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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