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Human RPS6KA1 Knockdown Cell Line (Wb-Validated) #C61167

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RT-qPCR analysis. HeLa cells were infected with RPS6KA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RPS6KA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RPS6KA1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    RPS6KA1
  • 货号：
    C61167
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human RPS6KA1 Knockdown Cell Line (Wb-Validated)
Aliases RPS6KA1; Ribosomal Protein S6 Kinase A1; RSK1; MAPKAPK1; HU-1; MAP Kinase-Activated Protein Kinase 1a; 90 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Alpha-1; MAPK-Activated Protein Kinase 1a; Ribosomal S6 Kinase 1; MAPKAP Kinase 1a; EC 2.7.11.1; MAPKAPK-1a; MAPKAPK1A; P90-RSK 1; P90RSK1; P90Rsk; P90S6K; RSK-1; DJ590P13.1 (Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1); Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1; S6K-Alpha 1; S6K-Alpha-1; EC 2.7.11; RSK(多P90RSK)
Background

Gene Name: RPS6KA1

NCBI Gene Entry: 6195
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human RPS6KA1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with RPS6KA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. RPS6KA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RPS6KA1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    RPS6KA1
  • 货号：
    C61167
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with RPS6KA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RPS6KA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RPS6KA1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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