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Human BMAL1 Knockdown Cell Line (Wb-Validated) #C63424

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RT-qPCR analysis. HeLa cells were infected with BMAL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. BMAL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against BMAL1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

BMAL1
货号：

C63424
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human BMAL1 Knockdown Cell Line (Wb-Validated)
Aliases	BMAL1; Basic Helix-Loop-Helix ARNT Like 1; BHLHe5; PASD3; MOP3; Brain And Muscle ARNT-Like 1; ARNTL1; ARNTL; JAP3; Aryl Hydrocarbon Receptor Nuclear Translocator-Like Protein 1; Aryl Hydrocarbon Receptor Nuclear Translocator Like; Basic Helix-Loop-Helix ARNT-Like Protein 1; Class E Basic Helix-Loop-Helix Protein 5; Basic Helix-Loop-Helix Family Member E5; Basic-Helix-Loop-Helix-PAS Protein MOP3; PAS Domain-Containing Protein 3; Member Of PAS Superfamily 3; PAS Domain Containing 3; Member Of PAS Protein 3; BHLH-PAS Protein JAP3; Mutant Basic Helix-Loop-Helix ARNT-Like Protein 1; Testis Tissue Sperm-Binding Protein Li 50e; Basic-Helix-Loop-Helix-PAS Orphan MOP3; ARNT-Like Protein 1, Brain And Muscle; BMAL1c; BHLHE5; TIC
Background	Gene Name: BMAL1 NCBI Gene Entry: 406
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human BMAL1 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with BMAL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. BMAL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against BMAL1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

BMAL1
货号：

C63424
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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