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Human GIPC1 Knockdown Cell Line (Wb-Validated) #C63585

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Western blotting analysis. GIPC1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against GIPC1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
RT-qPCR analysis. HT-1080 cells were infected with GIPC1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • 基因名：
    GIPC1
  • 货号：
    C63585
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human GIPC1 Knockdown Cell Line (Wb-Validated)
Aliases GIPC1; GIPC PDZ Domain Containing Family Member 1; TIP-2; GIPC; GLUT1CBP; SYNECTIN; RGS19IP1; Hs.6454; C19orf3; SEMCAP; NIP; Regulator Of G-Protein Signalling 19 Interacting Protein 1; PDZ Domain-Containing Protein GIPC1; GAIP C-Terminus-Interacting Protein; RGS-GAIP-Interacting Protein; RGS19-Interacting Protein 1; Tax Interaction Protein 2; GIPC PDZ Domain Containing Family, Member 1; IGF-1 Receptor Interacting Protein 1; Chromosome 19 Open Reading Frame 3; GLUT1 C-Terminal Binding Protein; SYNECTIIN; Synectin; IIP-1; OPDM2
Background

Gene Name: GIPC1

NCBI Gene Entry: 10755
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human GIPC1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • Western blotting analysis. GIPC1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against GIPC1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • RT-qPCR analysis. HT-1080 cells were infected with GIPC1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Citation(0)
  • 基因名：
    GIPC1
  • 货号：
    C63585
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
Western blotting analysis. GIPC1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against GIPC1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
RT-qPCR analysis. HT-1080 cells were infected with GIPC1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
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