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Human PSMB8 Knockdown Cell Line (Wb-Validated) #C63657

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RT-qPCR analysis. HT-1080 cells were infected with PSMB8-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. PSMB8 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PSMB8 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

PSMB8
货号：

C63657
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human PSMB8 Knockdown Cell Line (Wb-Validated)
Aliases	PSMB8; Proteasome 20S Subunit Beta 8; RING10; PSMB5i; D6S216E; LMP7; Multicatalytic Endopeptidase Complex Subunit C13; Really Interesting New Gene 10 Protein; Proteasome Subunit Beta Type-8; Low Molecular Mass Protein 7; Proteasome Subunit Beta 8; Proteasome Component C13; Macropain Subunit C13; EC 3.4.25.1; Beta5i; Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Peptidase 7); Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Protease 7); Proteasome (Prosome, Macropain) Subunit, Beta Type, 8; Large Multifunctional Peptidase 7; Proteasome Catalytic Subunit 3i; Low Molecular Weight Protein 7; Proteasome Subunit Beta 5i; Proteasome Subunit Beta-5i; Proteasome-Related Gene 7; Proteasome Subunit Β5i; Protease Component C13; Proteasome Subunit Y2; D6S216; PRAAS1; ALDD; NKJO; JMP; Y2
Background	Gene Name: PSMB8 NCBI Gene Entry: 5696
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human PSMB8 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HT-1080 cells were infected with PSMB8-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PSMB8 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PSMB8 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

PSMB8
货号：

C63657
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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