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Human TGM2 Knockdown Cell Line (Wb-Validated) #C63732

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RT-qPCR analysis. HT-1080 cells were infected with TGM2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. TGM2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against TGM2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

TGM2
货号：

C63732
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human TGM2 Knockdown Cell Line (Wb-Validated)
Aliases	TGM2; Transglutaminase 2; TGC; C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase; Protein-Glutamine Noradrenalinyltransferase TGM2; Protein-Glutamine Gamma-Glutamyltransferase 2; Protein-Glutamine Histaminyltransferase TGM2; Protein-Glutamine Dopaminyltransferase TGM2; Protein-Glutamine Serotonyltransferase TGM2; Protein-Glutamine Deamidase TGM2; Erythrocyte Transglutaminase; Tissue Transglutaminase; Transglutaminase II; Protein G Alpha(H); Transglutaminase C; Transglutaminase H; Isopeptidase TGM2; Heart G Alpha(H); HhG Alpha(H); EC 2.3.2.13; TGase II; TGase C; TGase H; TGase-2; TTgase; TG(C); G(H); HTG2; TG2; TTG; Transglutaminase 2 (C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase); Transglutaminase-2; EC 3.5.1.44; EC 3.4.-.-; EC 2.3.1.-
Background	Gene Name: TGM2 NCBI Gene Entry: 7052
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human TGM2 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HT-1080 cells were infected with TGM2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. TGM2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against TGM2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

TGM2
货号：

C63732
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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