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Human SUMO1 Knockdown Cell Line (Wb-Validated) #C63746

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RT-qPCR analysis. HeLa cells were infected with SUMO1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SUMO1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SUMO1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    SUMO1
  • 货号:
    C63746
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human SUMO1 Knockdown Cell Line (Wb-Validated)
Aliases SUMO1; Small Ubiquitin Like Modifier 1; SMT3H3; SMT3C; GMP1; SUMO-1; OFC10; PIC1; UBL1; Ubiquitin-Homology Domain Protein PIC1; Small Ubiquitin-Related Modifier 1; Ubiquitin-Like Protein SMT3C; Ubiquitin-Like Protein UBL1; SMT3 Homolog 3; Sentrin; SMT3 Suppressor Of Mif Two 3 Homolog 1 (S. Cerevisiae); SMT3 Suppressor Of Mif Two 3 Homolog 1 (Yeast); SMT3 Suppressor Of Mif Two 3 Homolog 1; Ubiquitin-Like 1 (Sentrin); GAP Modifying Protein 1; GAP-Modifying Protein 1; SENP2; Smt3C; DAP1; SMT3
Background

Gene Name: SUMO1

NCBI Gene Entry: 7341

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human SUMO1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with SUMO1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. SUMO1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SUMO1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with SUMO1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SUMO1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SUMO1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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