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Human BDH2 Knockdown Cell Line (Wb-Validated) #C63795

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RT-qPCR analysis. HeLa cells were infected with BDH2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. BDH2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against BDH2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    BDH2
  • 货号：
    C63795
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human BDH2 Knockdown Cell Line (Wb-Validated)
Aliases BDH2; 3-HydroxybutyRate Dehydrogenase 2; SDR15C1; PRO20933; UCPA-OR; UNQ6308; DHRS6; Short Chain Dehydrogenase/Reductase Family 15C Member 1; Dehydrogenase/Reductase SDR Family Member 6; 3-HydroxybutyRate Dehydrogenase, Type 2; (R)-Beta-HydroxybutyRate Dehydrogenase; 4-Oxo-L-Proline Reductase; Oxidoreductase UCPA; FLJ13261; Short Chain Dehydrogenase/Reductase Family 15C, Member 1; Dehydrogenase/Reductase (SDR Family) Member 6; 3-HydroxybutyRate Dehydrogenase Type 2; R-Beta-HydroxybutyRate Dehydrogenase; EC 1.1.1.104; EC 1.1.1.30; EC 1.1.1.-; EFA6R
Background

Gene Name: BDH2

NCBI Gene Entry: 56898
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human BDH2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with BDH2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. BDH2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against BDH2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    BDH2
  • 货号：
    C63795
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with BDH2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. BDH2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against BDH2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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