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Human CFAP298 Knockdown Cell Line (Wb-Validated) #C63796

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RT-qPCR analysis. HT-1080 cells were infected with CFAP298-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CFAP298 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CFAP298 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    CFAP298
  • 货号:
    C63796
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human CFAP298 Knockdown Cell Line (Wb-Validated)
Aliases CFAP298; Cilia And Flagella Associated Protein 298; C21orf48; C21orf59; DNAAF16; CILD26; FBB18; Kur; Cilia- And Flagella-Associated Protein 298; Dynein Axonemal Assembly Factor 16; Protein Kurly Homolog; FLJ20467; Prostate Cancer Upregulated Protein 1; Chromosome 21 Open Reading Frame 48; Chromosome 21 Open Reading Frame 59; Kurly Homolog (Zebrafish); UPF0769 Protein C21orf59; Kurly Homolog
Background

Gene Name: CFAP298

NCBI Gene Entry: 56683

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human CFAP298 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with CFAP298-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. CFAP298 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CFAP298 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HT-1080 cells were infected with CFAP298-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CFAP298 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CFAP298 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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