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Human RAP1B Knockdown Cell Line (Wb-Validated) #C65217

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RT-qPCR analysis. HT-1080 cells were infected with RAP1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. RAP1B protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RAP1B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

RAP1B
货号：

C65217
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human RAP1B Knockdown Cell Line (Wb-Validated)
Aliases	RAP1B; RAP1B, Member Of RAS Oncogene Family; K-REV; RAL1B; GTP-Binding Protein Smg P21B; Ras-Related Protein Rap-1b; DKFZp586H0723; Ras Family Small GTP Binding Protein RAP1B; RAS-Related Protein RAP1B; Small GTP Binding Protein; EC 3.6.5.2; THC11
Background	Gene Name: RAP1B NCBI Gene Entry: 5908
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human RAP1B Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HT-1080 cells were infected with RAP1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RAP1B protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RAP1B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

RAP1B
货号：

C65217
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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