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Human RNF40 Knockdown Cell Line (Wb-Validated) #C62492

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RT-qPCR analysis. HeLa cells were infected with RNF40-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. RNF40 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against RNF40 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

RNF40
货号：

C62492
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human RNF40 Knockdown Cell Line (Wb-Validated)
Aliases	RNF40; Ring Finger Protein 40; RBP95; BRE1B; KIAA0661; STARING; 95 KDa Retinoblastoma-Associated Protein; RING-Type E3 Ubiquitin Transferase BRE1B; E3 Ubiquitin-Protein Ligase BRE1B; BRE1-B; Ring Finger Protein 40, E3 Ubiquitin Protein Ligase; BRE1 E3 Ubiquitin Ligase Homolog B (S. Cerevisiae); 95 KDa Retinoblastoma Protein Binding Protein; BRE1 E3 Ubiquitin Ligase Homolog B; RING Finger Protein 40; Rb-Associated Protein; EC 2.3.2.27; EC 6.3.2
Background	Gene Name: RNF40 NCBI Gene Entry: 9810
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human RNF40 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with RNF40-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RNF40 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against RNF40 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

RNF40
货号：

C62492
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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