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Human NR2F2 Knockdown Cell Line (Wb-Validated) #C65652

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RT-qPCR analysis. HeLa cells were infected with NR2F2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.NR2F2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NR2F2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    NR2F2
  • 货号：
    C65652
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human NR2F2 Knockdown Cell Line (Wb-Validated)
Aliases NR2F2; Nuclear Receptor Subfamily 2 Group F Member 2; COUP Transcription Factor II; COUPTFB; COUPTF2; TFCOUP2; SVP40; NF-E3; ARP1; Apolipoprotein A-I Regulatory Protein 1; COUP Transcription Factor 2; COUP-TFII; ARP-1; Chicken Ovalbumin Upstream Promoter Transcription Factor 2; Chicken Ovalbumin Upstream Promoter-Transcription Factor I; Nuclear Receptor Subfamily 2, Group F, Member 2; ADP-Ribosylation Factor Related Protein 1; Apolipoprotein AI Regulatory Protein 1; COUP-TF II; COUPTFII; COUP-TF2; CHTD4; SRXX5
Background

Gene Name: NR2F2

NCBI Gene Entry: 7026
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human NR2F2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with NR2F2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.NR2F2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NR2F2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    NR2F2
  • 货号：
    C65652
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with NR2F2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.NR2F2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NR2F2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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