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Human AGO3 Knockdown Cell Line (Wb-Validated) #C68651

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RT-qPCR analysis. HT-1080 cells were infected with AGO3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. AGO3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AGO3 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

AGO3
货号：

C68651
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human AGO3 Knockdown Cell Line (Wb-Validated)
Aliases	AGO3; Argonaute RISC Catalytic Component 3; EIF2C3; HAGO3; Eukaryotic Translation Initiation Factor 2C, 3; Protein Argonaute-3; FLJ12765; Eukaryotic Translation Initiation Factor 2C 3; Argonaute 3, RISC Catalytic Component; EC 3.1.26.N2; Argonaute 3; Argonaute3; EIF-2C 3; EIF2C 3; HAgo3
Background	Gene Name: AGO3 NCBI Gene Entry: 192669
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human AGO3 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HT-1080 cells were infected with AGO3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AGO3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AGO3 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

AGO3
货号：

C68651
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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