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Human ATG7 Knockdown Cell Line (Wb-Validated) #C68737

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RT-qPCR analysis. 293T cells were infected with ATG7-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ATG7 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ATG7 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    ATG7
  • 货号：
    C68737
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

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Information
Product Name Human ATG7 Knockdown Cell Line (Wb-Validated)
Aliases ATG7; Autophagy Related 7; Ubiquitin-Activating Enzyme E1-Like Protein; Ubiquitin-Like Modifier-Activating Enzyme ATG7; ATG12-Activating Enzyme E1 ATG7; APG7L; HAGP7; ATG7 Autophagy Related 7 Homolog (S. Cerevisiae); APG7 Autophagy 7-Like (S. Cerevisiae); Autophagy-Related Protein 7; APG7 Autophagy 7-Like; APG7-LIKE; APG7-Like; GSA7
Background

Gene Name: ATG7

NCBI Gene Entry: 10533
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human ATG7 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. 293T cells were infected with ATG7-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. ATG7 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ATG7 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    ATG7
  • 货号：
    C68737
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. 293T cells were infected with ATG7-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ATG7 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ATG7 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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