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Human ALKBH1 Knockdown Cell Line (Wb-Validated) #C69441

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RT-qPCR analysis. HeLa cells were infected with ALKBH1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ALKBH1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies against ALKBH1 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    ALKBH1
  • 货号：
    C69441
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human ALKBH1 Knockdown Cell Line (Wb-Validated)
Aliases ALKBH1; AlkB Homolog 1, Histone H2A Dioxygenase; ABH; ALKBH; HABH; Alpha-KetoglutaRate-Dependent Dioxygenase ABH1; Alkylated DNA Repair Protein AlkB Homolog 1; DNA N6-Methyl Adenine Demethylase ALKBH1; MRNA N(3)-Methylcytidine Demethylase; DNA Oxidative Demethylase ALKBH1; Nucleic Acid Dioxygenase ALKBH1; DNA 6mA Demethylase; DNA Lyase ABH1; AlkB; ABH1; AlkB, Alkylation Repair Homolog 1 (E. Coli); AlkB, Alkylation Repair Homolog (E. Coli); TRNA N1-Methyl Adenine Demethylase; AlkB, Alkylation Repair Homolog 1; Alkylation Repair, AlkB Homolog; DNA Demethylase ALKBH1; EC 1.14.11.51; EC 1.14.11.33; EC 1.14.11.-; EC 4.2.99.18; ALKB
Background

Gene Name: ALKBH1

NCBI Gene Entry: 8846
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human ALKBH1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with ALKBH1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. ALKBH1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies against ALKBH1 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    ALKBH1
  • 货号：
    C69441
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with ALKBH1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ALKBH1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies against ALKBH1 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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