Recombinant Antibody/shRNA Knockdown Validated

KD-Validated Anti-ATG7 Rabbit Monoclonal Antibody #61177

基因名：
ATG7
货号：
61177
应用：
Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
物种反应性：
Human, Mouse, Rat
Western blotting analysis using anti-ATG7 antibody (Cat#61177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG7 antibody (Cat#61177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ATG7 antibody (Cat#61177). ATG7 expression in wild type (WT) and ATG7 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ATG7 antibody (Cat#61177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ATG7 expression in HT-1080 cells using ATG7 antibody (Cat#61177, 1:2,000). Green, isotype control; red, ATG7.
Immunocytochemical staining of HT-1080 cells with ATG7 antibody (Cat#61177, 1:1,000). Nuclei were stained blue with DAPI; ATG7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Information

Aliases:
ATG7; Autophagy Related 7; Ubiquitin-Activating Enzyme E1-Like Protein; Ubiquitin-Like Modifier-Activating Enzyme ATG7; ATG12-Activating Enzyme E1 ATG7; APG7L; HAGP7; ATG7 Autophagy Related 7 Homolog (S. Cerevisiae); APG7 Autophagy 7-Like (S. Cerevisiae); Autophagy-Related Protein 7; APG7 Autophagy 7-Like; APG7-LIKE; APG7-Like; GSA7


Background:
UniProt Entry: O95352; NCBI Gene Entry: 10533


Application Information
Molecular Weight: Predicted, 78 kDa; observed, 70 kDa
Clonality: Rabbit monoclonal antibody
Clone ID: 24GB6075
Species Reactivity: Human, Mouse, Rat
Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)


Immunogen
A synthesized peptide derived from human Atg7 (Apg7)


Isotype
Rabbit IgG


Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.


Storage
Store at -20 °C for one year.


Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000


Protocols
For general and specific antibody protocols please visit our website. Read all instructions before using this product.


Size Price
20 μL ¥ 650.00
50 μL ¥ 1350.00
100 μL ¥ 2500.00
Datasheet
MSDS

RECENTLY VIEWED

Note

Product Datasheet
For research use only

BETTER ROOTS BETTER FRUITS

KD-Validated Anti-ATG7 Rabbit Monoclonal Antibody #61177
Recombinant Antibody/shRNA Knockdown Validated

Aliases 

ATG7; Autophagy Related 7; Ubiquitin-Activating Enzyme E1-Like Protein; Ubiquitin-Like Modifier-Activating Enzyme ATG7; ATG12-Activating Enzyme E1 ATG7; APG7L; HAGP7; ATG7 Autophagy Related 7 Homolog (S. Cerevisiae); APG7 Autophagy 7-Like (S. Cerevisiae); Autophagy-Related Protein 7; APG7 Autophagy 7-Like; APG7-LIKE; APG7-Like; GSA7


Background 

UniProt Entry: O95352; NCBI Gene Entry: 10533


Application Information 

Molecular Weight: Predicted, 78 kDa; observed, 70 kDa
Clonality: Rabbit monoclonal antibody
Clone ID: 24GB6075
Species Reactivity: Human, Mouse, Rat
Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)


Immunogen 

A synthesized peptide derived from human Atg7 (Apg7)


Isotype 

Rabbit IgG


Storage Buffer 

Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.


Storage 

Store at -20 °C for one year.


Recommended Dilutions 

Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000


Protocols 

For general and specific antibody protocols please visit our website. Read all instructions before using this product.






Antibody Validation 

Western blotting analysis using anti-ATG7 antibody (Cat#61177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG7 antibody (Cat#61177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Antibody Validation 

Western blotting analysis using anti-ATG7 antibody (Cat#61177). ATG7 expression in wild type (WT) and ATG7 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ATG7 antibody (Cat#61177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Antibody Validation 

Flow cytometric analysis of ATG7 expression in HT-1080 cells using ATG7 antibody (Cat#61177, 1:2,000). Green, isotype control; red, ATG7.

Antibody Validation 

Immunocytochemical staining of HT-1080 cells with ATG7 antibody (Cat#61177, 1:1,000). Nuclei were stained blue with DAPI; ATG7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Hefei SciBen Biotech Co., Ltd

Building 6, 98 Jiulong Road, Hefei, Anhui 230601, China

Tel: 0551-163635518 E-mail: support@scibenbio.com www.scibenbio.com

Material Safety Data Sheet (MSDS) for Antibody

I. Identification:

Catalog Number: 61177

Product Name: KD-Validated Anti-ATG7 Rabbit Monoclonal Antibody #61177

Manufacturer & Supplier: Hefei SciBen Biotech Co., Ltd

Address:Building 6, 98 Jiulong Road, Hefei, Anhui 230601, China

Tel: 0551-63635518

E-mail:support@scibenbio.com

Web: www.scibenbio.com

II. Composition/Information on Ingredients:

This product is composed of antibodies in aqueous buffer solution. According to 29

CFR1910.1200(d), hazardous ingredients at less than < 1% and carcinogens at less than < 0.1% are considered non-hazardous. Any hazardous or carcinogenic ingredients exceeding these criteria are listed below.

This product may contain the following hazardous ingredients.

Ingredient
CAS#
Percent
glycerol
56-81-5
50%

III. Hazard Identification:

Emergency Overview of Hazardous ingredient:glycerol (CAS# 56-81-5)

Caution:Avoid contact and inhalation.

Target Organ: Kidneys.

NFPA Rating:

Health Rating: 1

Flammability Rating: 0

Reactivity Rating:0

IV. First Aid Measures:

Inhalation:If inhaled, remove to fresh air. If breathing is difficult, get medical attention.

Ingestion: If swallowed and person is conscious, rinse out mouth with water. Get medical attention.

Skin Exposure:In case of contact, wash skin with soap and water.

Eye Exposure:In case of contact with eyes, immediately flush eyes water for at least 5minutes. Get medical attention.

V. Fire Fighting Measures:

Flash Point: Data not available.

Autoignition Temperature: Data not available.

Fire Extinguishing Media:Water spray, dry chemical, foam, or carbon dioxide.

Firefighting:Wear protective clothing and self-contained breathing apparatus to prevent contact with skin and eyes.

VI. Accidental Release Measures:

Absorb liquid with an absorbent material. Transfer contaminated absorbent to a chemical waste container for disposal.

VII. Handling And Storage:

Avoid inhalation and contact with eyes and skin. Avoid prolonged or repeated exposure. Store at –20 °C in tightly closed container.


VIII. Exposure Controls/Personal Protection:

Engineering Controls: Maintain adequate ventilation, eye wash and quick-drench facilities

IX. Physical and Chemical Properties:

Physical State:Colorless liquid.

Odor:Odorless.

Boiling Point:Data not available.

Melting Point:Data not available.

Volatile Organic Compound:Data not available.

Solubility in water:Readily miscible in water.

X. Stability and Reactivity:

Stability:Stable.

Hazardous Decomposition:May form carbon dioxide and carbon monoxide.

Conditions to avoid:Strong oxidizing agents

XI. Toxicological Information:

May cause skin irritation.

May be toxic if absorbed through skin or ingested.

May cause eye irritation.

Target Organs: Kidneys

Prolonged exposure may cause nausea, headache, and vomiting.

XII. Ecological Information:

Data not available.

XIII. Disposal Considerations:

Dispose of in accordance with federal, state and local environmental regulations.

XIV. Transport Information:

D.O.T.: This substance is considered non-hazardous for transport.

IATA: This substance is considered non-hazardous for air transport.

XV. Regulatory Information:

EU Regulation/Classification/Labeling Information: Not available for this product.

Chemical Inventory Status:

SARA Listed Component: None.

TSCA Listed Component: None.

Canada (WHMIS): DSL No, NDSL No.

XVI. Other Information:

This product is sold only for research use by personnel familiar with chemicals and who are well trained in good laboratory habits, such as avoiding spills, keeping hands clean at all times and not rubbing eyes with hands while working in the laboratory.

This product is sold only in microliter quantities for use in life sciences research. No other use is intended, and any other use may involve substantive hazards.

The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide for experienced personnel. Hefei SciBen Biotech Co., Ltd shall not be held liable for any damage resulting from the handling of or from contact with the above product. The burden of safe use of this material rests entirely with the user.


Issuing Date: 8/16/2024 Version: 1.0

Hefei SciBen Biotech Co., Ltd provides the information contained herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. Hefei SciBen Biotech Co., Ltd makes no representations or warranties, either express or implied, including without limitation any warranties of merchantability, fitness for a particular purpose with respect to the information set forth herein or the product to which the information refers. Accordingly, Hefei SciBen Biotech Co., Ltd will not be responsible for damages resulting from use of or reliance upon this information.