Anti-Phospho-A-RAF (Y302) Rabbit Polyclonal Antibody #U0152

基因名：
ARAF
货号：
U0152
应用：
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (IC)
物种反应性：
Human, Mouse, Rat, Bovine, Pig, Sheep
Immunofluorescent analysis of A-RAF (Phospho-Y302) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of A-RAF (Phospho-Y302) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of A-RAF (Phospho-Y302) expression in HeLa PMA-treated (A), Raji (B), HT29 (C), NIH3T3 (D), SP2/0 PMA-treated (E), PC12 PMA-treated (F) whole cell lysates.

Information

Aliases:
ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks


Background:
UniProt Entry: P10398; NCBI Gene Entry: 369


Application Information
Molecular Weight: Predicted, 67 kD; Observed, 68 kD
Clonality: Rabbit polyclonal antibody
Species Reactivity: Human, mouse, rat, bovine, pig, sheep
Applications Tested: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (IC)


Immunogen
A synthesized peptide derived from human Phospho-A-RAF (Y302)


Isotype
Rabbit IgG


Storage Buffer
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.


Storage
Store at -20 °C for one year.


Recommended Dilutions
Western Blotting (WB):1:500-1:1,000
Immunohistochemistry (IH): 1:50-1:100
Immunofluorescence (IF)/Immunocytochemistry (IC): 1:50-1:200


Protocols
For general and specific antibody protocols please visit our website. Read all instructions before using this product.


Size Price
20 μL ¥ 560.00
50 μL ¥ 1100.00
100 μL ¥ 2000.00
Datasheet
MSDS

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Note

Product Datasheet
For research use only

BETTER ROOTS BETTER FRUITS

Anti-Phospho-A-RAF (Y302) Rabbit Polyclonal Antibody #U0152


Aliases

ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks


Background

UniProt Entry: P10398; NCBI Gene Entry: 369


Application Information

Molecular Weight: Predicted, 67 kD; Observed, 68 kD
Clonality: Rabbit polyclonal antibody
Species Reactivity: Human, mouse, rat, bovine, pig, sheep
Applications Tested: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (IC)


Immunogen

A synthesized peptide derived from human Phospho-A-RAF (Y302)


Isotype

Rabbit IgG


Storage Buffer

Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.


Storage

Store at -20 °C for one year.


Recommended Dilutions

Western Blotting (WB):1:500-1:1,000
Immunohistochemistry (IH): 1:50-1:100
Immunofluorescence (IF)/Immunocytochemistry (IC): 1:50-1:200


Protocols

For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Antibody Validation

Immunofluorescent analysis of A-RAF (Phospho-Y302) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Antibody Validation

Immunohistochemical analysis of A-RAF (Phospho-Y302) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Antibody Validation

Western blotting analysis of A-RAF (Phospho-Y302) expression in HeLa PMA-treated (A), Raji (B), HT29 (C), NIH3T3 (D), SP2/0 PMA-treated (E), PC12 PMA-treated (F) whole cell lysates.

GenuIN Biotech LLC

2000 Kraft Drive, Suite 1110, Blacksburg, VA 24060, USA

Tel: 0551-163635518 Fax: 540.605.9771

E-mail: info@genuinbiotech.com www.genuinbiotech.com

Material Safety Data Sheet (MSDS) for Antibody

I. Identification:

Catalog Number: U0152

Product Name: Anti-Phospho-A-RAF (Y302) Rabbit Polyclonal Antibody #U0152

Manufacturer & Supplier: Hefei SciBen Biotech Co., Ltd

Address:Building 6, 98 Jiulong Road, Hefei, Anhui 230601, China

Tel: 0551-63635518

Fax: 540.605.9771

E-mail:support@scibenbio.com

II. Composition/Information on Ingredients:

This product is composed of antibodies in aqueous buffer solution. According to 29

CFR1910.1200(d), hazardous ingredients at less than < 1% and carcinogens at less than < 0.1% are considered non-hazardous. Any hazardous or carcinogenic ingredients exceeding these criteria are listed below.

This product may contain the following hazardous ingredients.

Ingredient:glycerol

CAS#:56-81-5

Percent: 50%

III. Hazard Identification:

Emergency Overview of Hazardous ingredient:glycerol (CAS# 56-81-5)

Caution:Avoid contact and inhalation.

Target Organ: Kidneys.

NFPA Rating:

Health Rating: 1

Flammability Rating: 0

Reactivity Rating:0

IV. First Aid Measures:

Inhalation:If inhaled, remove to fresh air. If breathing is difficult, get medical attention.

Ingestion: If swallowed and person is conscious, rinse out mouth with water. Get medical attention.

Skin Exposure:In case of contact, wash skin with soap and water.

Eye Exposure:In case of contact with eyes, immediately flush eyes water for at least 5minutes. Get medical attention.

V. Fire Fighting Measures:

Flash Point: Data not available.

Autoignition Temperature: Data not available.

Fire Extinguishing Media:Water spray, dry chemical, foam, or carbon dioxide.

Firefighting:Wear protective clothing and self-contained breathing apparatus to prevent contact with skin and eyes.

VI. Accidental Release Measures:

Absorb liquid with an absorbent material. Transfer contaminated absorbent to a chemical waste container for disposal.

VII. Handling And Storage:

Avoid inhalation and contact with eyes and skin. Avoid prolonged or repeated exposure. Store at –20 °C in tightly closed container.


VIII. Exposure Controls/Personal Protection:

Engineering Controls: Maintain adequate ventilation, eye wash and quick-drench facilities

IX. Physical and Chemical Properties:

Physical State:Colorless liquid.

Odor:Odorless.

Boiling Point:Data not available.

Melting Point:Data not available.

Volatile Organic Compound:Data not available.

Solubility in water:Readily miscible in water.

X. Stability and Reactivity:

Stability:Stable.

Hazardous Decomposition:May form carbon dioxide and carbon monoxide.

Conditions to avoid:Strong oxidizing agents

XI. Toxicological Information:

May cause skin irritation.

May be toxic if absorbed through skin or ingested.

May cause eye irritation.

Target Organs: Kidneys

Prolonged exposure may cause nausea, headache, and vomiting.

XII. Ecological Information:

Data not available.

XIII. Disposal Considerations:

Dispose of in accordance with federal, state and local environmental regulations.

XIV. Transport Information:

D.O.T.: This substance is considered non-hazardous for transport.

IATA: This substance is considered non-hazardous for air transport.

XV. Regulatory Information:

EU Regulation/Classification/Labeling Information: Not available for this product.

Chemical Inventory Status:

SARA Listed Component: None.

TSCA Listed Component: None.

Canada (WHMIS): DSL No, NDSL No.

XVI. Other Information:

This product is sold only for research use by personnel familiar with chemicals and who are well trained in good laboratory habits, such as avoiding spills, keeping hands clean at all times and not rubbing eyes with hands while working in the laboratory. This product is sold only in microliter quantities for use in life sciences research. No other use is intended, and any other use may involve substantive hazards. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide for experienced personnel. Hefei SciBen Biotech Co., Ltd shall not be held liable for any damage resulting from the handling of or from contact with the above product. The burden of safe use of this material rests entirely with the user.

Issuing Date: 8/16/2024 Version: 1.0

GenuIN Biotech LLC provides the information contained herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. GenuIN Biotech LLC makes no representations or warranties, either express or implied, including without limitation any warranties of merchantability, fitness for a particular purpose with respect to the information set forth herein or the product to which the information refers. Accordingly, GenuIN Biotech LLC will not be responsible for damages resulting from use of or reliance upon this information.