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Anti-GFP Tag Mouse Monoclonal Antibody, HRP conjugated #T031-HRP

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Western blotting analysis using anti-GFP-tag antibody (Cat#T031-HRP). GFP tagged-fusion protein (0.50 μg) expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-GFP-tag antibody (Cat#T031-HRP, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
  • 货号:
    T031-HRP
  • 应用:
    WB
  • 物种反应性:
    Recombinant protein
规格:
20 μL,50 μL,100 μL
价格:
¥ 380.00,¥ 750.00,¥ 1,350.00
Information
Product Name Anti-GFP Tag Mouse Monoclonal Antibody, HRP conjugated
Aliases GFP; yfp; Green fluorescent protein
Background The GFP (Green Fluorescent Protein) tag is a 27 kDa fluorescent protein derived from the jellyfish Aequorea victoria. It emits bright green light (λ~509 nm) upon exposure to blue or UV light, enabling real-time visualization of tagged proteins in live or fixed cells without additional substrates. Widely used as a reporter gene, GFP-tagging allows non-invasive tracking of protein localization, expression dynamics, and interactions across diverse organisms (e.g., bacteria, mammalian cells). Its engineered variants (e.g., EGFP, mCherry) offer enhanced brightness, photostability, and spectral versatility for multiplex imaging.
Application Information

Molecular Weight: Recombinant protein dependent

Clonality: Mouse monoclonal antibody

Species Reactivity: Recombinant protein

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Conjugation: HRP

Immunogen Recombinant protein of GFP-tag
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH7.2) containing 50% glycerol, 1% BSA and 0.02% Proclin300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:5,000
Immunocytochemistry (IC): 1:500-1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-GFP-tag antibody (Cat#T031-HRP). GFP tagged-fusion protein (0.50 μg) expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-GFP-tag antibody (Cat#T031-HRP, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Western blotting analysis using anti-GFP-tag antibody (Cat#T031-HRP). GFP tagged-fusion protein (0.50 μg) expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-GFP-tag antibody (Cat#T031-HRP, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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