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Anti-Phospho-Annexin A2 (S26) Rabbit Polyclonal Antibody #U0352

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Immunocytochemical analysis of Annexin A2 (Phospho-S26) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of Annexin A2 (Phospho-S26) expression in mouse lung (A), rat lung (B), rat liver (C) whole cell lysates.
  • 基因名:
    ANXA2
  • 货号:
    U0352
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R, B, D, Mk, S
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Phospho-Annexin A2 (S26) Rabbit Polyclonal Antibody
Aliases ANX2; ANX2L4; CAL1H; LPC2D; Annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; Protein I; p36
Background

Gene Name: ANXA2

NCBI Gene Entry: 302

UniProt Entry: P07355

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 38 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, dog, monkey, sheep

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Phospho-Annexin A2 (S26)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of Annexin A2 (Phospho-S26) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of Annexin A2 (Phospho-S26) expression in mouse lung (A), rat lung (B), rat liver (C) whole cell lysates.
Citation(0)
Immunocytochemical analysis of Annexin A2 (Phospho-S26) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of Annexin A2 (Phospho-S26) expression in mouse lung (A), rat lung (B), rat liver (C) whole cell lysates.
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