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Anti-Phospho-ATP1A1 (S16) Rabbit Polyclonal Antibody #U0540

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Immunohistochemical analysis of ATP1A1 (Phospho-S16) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of ATP1A1 (Phospho-S16) expression in HEK293T (A), HCT116 (B), Panc1 (C), PC12 (D), CT26 (E) whole cell lysates.
  • 基因名:
    ATP1A1
  • 货号:
    U0540
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R, B, D, P, Rb, S
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Phospho-ATP1A1 (S16) Rabbit Polyclonal Antibody
Aliases Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1
Background

Gene Name: ATP1A1

NCBI Gene Entry: 476

UniProt Entry: P05023

Application Information

Molecular Weight: Predicted, 112 kDa; observed, 112 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, dog, pig, rabbit, sheep

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Phospho-ATP1A1 (S16)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:50-1:100
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of ATP1A1 (Phospho-S16) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of ATP1A1 (Phospho-S16) expression in HEK293T (A), HCT116 (B), Panc1 (C), PC12 (D), CT26 (E) whole cell lysates.
Citation(0)
Immunohistochemical analysis of ATP1A1 (Phospho-S16) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of ATP1A1 (Phospho-S16) expression in HEK293T (A), HCT116 (B), Panc1 (C), PC12 (D), CT26 (E) whole cell lysates.
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