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KD-Validated Anti-PIK3C2A Mouse Monoclonal Antibody #65052

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Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-PIK3C2A antibody (Cat#65052, 1:1,000) . Nuclei were stained blue with DAPI; PIK3C2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PIK3C2A expression in HepG2 cells using anti-PIK3C2A antibody (Cat#65052, 1:1,000). Green, isotype control; red, PIK3C2A.
  • 基因名：
    PIK3C2A
  • 货号：
    65052
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-PIK3C2A Mouse Monoclonal Antibody
Aliases PIK3C2A; Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Alpha; Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha; PI3K-C2alpha; Phosphoinositide-3-Kinase, Class 2, Alpha Polypeptide; Phosphoinositide 3-Kinase-C2-Alpha; PtdIns-3-Kinase C2 Subunit Alpha; PI3K-C2-Alpha; EC 2.7.1.154; Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha; Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 Alpha; C2-Containing Phosphatidylinositol Kinase; PI3-K-C2(ALPHA); EC 2.7.1.137; EC 2.7.1.153; PI3-K-C2A; EC 2.7.1; OCSKD; CPK
Background

Gene Name: PIK3C2A

NCBI Gene Entry: 5286

UniProt Entry: O00443
Application Information

Molecular Weight: Predicted, 191 kDa; observed, 191 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14050

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen Recombinant protein of human PIK3C2A
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with anti-PIK3C2A antibody (Cat#65052, 1:1,000) . Nuclei were stained blue with DAPI; PIK3C2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PIK3C2A expression in HepG2 cells using anti-PIK3C2A antibody (Cat#65052, 1:1,000). Green, isotype control; red, PIK3C2A.
Citation(0)
  • 基因名：
    PIK3C2A
  • 货号：
    65052
  • 应用：
    WB, IC, FCM, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-PIK3C2A antibody (Cat#65052, 1:1,000) . Nuclei were stained blue with DAPI; PIK3C2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PIK3C2A expression in HepG2 cells using anti-PIK3C2A antibody (Cat#65052, 1:1,000). Green, isotype control; red, PIK3C2A.
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