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Anti-Phospho-EPHA2 (Y588/596) Rabbit Polyclonal Antibody #U0628

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Immunocytochemical analysis of EPHA2 (Phospho-Y588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of EPHA2 (Phospho-Y588/596) expression in A431 (A), mouse brain (B) whole cell lysates.
  • 基因名：
    EPHA2/3/4
  • 货号：
    U0628
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, C, Mk
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-EPHA2 (Y588/596) Rabbit Polyclonal Antibody
Aliases EPHA2; ECK; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA3; ETK; ETK1; HEK; TYRO4; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1; EPHA4; HEK8; SEK; TYRO1; Ephrin type-A receptor 4; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK
Background

Gene Name: EPHA2/3/4

NCBI Gene Entry: 1969/2042/2043

UniProt Entry: P29317/P29320/P54764
Application Information

Molecular Weight: Predicted, 108, 110, 109 kDa; observed, 130 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, chicken, monkey

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-EPHA2 (Y588/596)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:100-1:500
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of EPHA2 (Phospho-Y588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of EPHA2 (Phospho-Y588/596) expression in A431 (A), mouse brain (B) whole cell lysates.
Citation(0)
  • 基因名：
    EPHA2/3/4
  • 货号：
    U0628
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, C, Mk
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunocytochemical analysis of EPHA2 (Phospho-Y588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of EPHA2 (Phospho-Y588/596) expression in A431 (A), mouse brain (B) whole cell lysates.
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