Flow cytometric analysis of High mobility group box 1 expression in HepG2 cells using High mobility group box 1 antibody (Cat#1114, 1:2,000). Green, isotype control; red, High mobility group box 1.
Immunocytochemical staining of HepG2 cells with High mobility group box 1 antibody (Cat#1114, 1:1,000). Nuclei were stained blue with DAPI; High mobility group box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-High mobility group box 1 antibody (Cat#1114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-High mobility group box 1 antibody (Cat#1114, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Anti-High mobility group box 1 Rabbit Monoclonal Antibody
Aliases
HMGB1; High Mobility Group Box 1; SBP-1; HMG3; HMG1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate; Binding Protein; High Mobility Group Protein B1; High Mobility Group Protein 1; Amphoterin; HMG-1; High-Mobility Group Box 1; DKFZp686A04236; DKFZP686A04236
Flow cytometric analysis of High mobility group box 1 expression in HepG2 cells using High mobility group box 1 antibody (Cat#1114, 1:2,000). Green, isotype control; red, High mobility group box 1.
Immunocytochemical staining of HepG2 cells with High mobility group box 1 antibody (Cat#1114, 1:1,000). Nuclei were stained blue with DAPI; High mobility group box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-High mobility group box 1 antibody (Cat#1114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-High mobility group box 1 antibody (Cat#1114, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of High mobility group box 1 expression in HepG2 cells using High mobility group box 1 antibody (Cat#1114, 1:2,000). Green, isotype control; red, High mobility group box 1.
Immunocytochemical staining of HepG2 cells with High mobility group box 1 antibody (Cat#1114, 1:1,000). Nuclei were stained blue with DAPI; High mobility group box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-High mobility group box 1 antibody (Cat#1114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-High mobility group box 1 antibody (Cat#1114, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
