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Anti-DDAH2 Rabbit Monoclonal Antibody #1428

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Flow cytometric analysis of DDAH2 expression in C2C12 cells using DDAH2 antibody (Cat#1428, 1:2000). Green, isotype control; red, DDAH2.
Immunocytochemical staining of C2C12 cells with DDAH2 antibody (Cat#1428, 1:1,000). Nuclei were stained blue with DAPI; DDAH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-DDAH2 antibody (Cat#1428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDAH2 antibody (Cat#1428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    DDAH2
  • 货号:
    1428
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-DDAH2 Rabbit Monoclonal Antibody
Aliases DDAH2; DDAH Family Member 2, ADMA-Independent; Dimethylarginine Dimethylaminohydrolase; DDAHII; G6A; N(G),N(G)-Dimethylarginine Dimethylaminohydrolase; Dimethylargininase-2; S-Phase Protein; EC 3.5.3.18; DDAH; NG30; Dimethylarginine Dimethylaminohydrolase II; Testis Tissue Sperm-Binding Protein Li 54e; Epididymis Secretory Protein Li 277; Protein G6a; HEL-S-277; DDAH-2; G6a
Background

Gene Name: DDAH2

NCBI Gene Entry: 23564

UniProt Entry: O95865

Application Information

Molecular Weight: Predicted, 30 kDa; observed, 30 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3820

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human DDAH2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of DDAH2 expression in C2C12 cells using DDAH2 antibody (Cat#1428, 1:2000). Green, isotype control; red, DDAH2.
  • Immunocytochemical staining of C2C12 cells with DDAH2 antibody (Cat#1428, 1:1,000). Nuclei were stained blue with DAPI; DDAH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-DDAH2 antibody (Cat#1428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDAH2 antibody (Cat#1428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of DDAH2 expression in C2C12 cells using DDAH2 antibody (Cat#1428, 1:2000). Green, isotype control; red, DDAH2.
Immunocytochemical staining of C2C12 cells with DDAH2 antibody (Cat#1428, 1:1,000). Nuclei were stained blue with DAPI; DDAH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-DDAH2 antibody (Cat#1428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDAH2 antibody (Cat#1428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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