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Anti-FLAP Rabbit Monoclonal Antibody #1653

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Flow cytometric analysis of FLAP expression in HeLa cells using FLAP antibody (Cat#1653, 1:2,000). Green, isotype control; red, FLAP.

Immunocytochemical staining of HeLa cells with FLAP antibody (Cat#1653, 1:1,000). Nuclei were stained blue with DAPI; FLAP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-FLAP antibody (Cat#1653). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLAP antibody (Cat#1653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ALOX5AP
货号：

1653
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-FLAP Rabbit Monoclonal Antibody
Aliases	ALOX5AP; Arachidonate 5-Lipoxygenase Activating Protein; FLAP; Arachidonate 5-Lipoxygenase-Activating Protein; MK-886-Binding Protein; Five-Lipoxygenase Activating Protein
Background	Gene Name: ALOX5AP NCBI Gene Entry: 241 UniProt Entry: P20292
Application Information	Molecular Weight: Predicted, 18 kDa; observed, 16 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3940 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human FLAP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of FLAP expression in HeLa cells using FLAP antibody (Cat#1653, 1:2,000). Green, isotype control; red, FLAP.
Immunocytochemical staining of HeLa cells with FLAP antibody (Cat#1653, 1:1,000). Nuclei were stained blue with DAPI; FLAP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-FLAP antibody (Cat#1653). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLAP antibody (Cat#1653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ALOX5AP
货号：

1653
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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