KD-Validated Anti-RALY Mouse Oligoclonal Antibody - 产品中心

KD-Validated Anti-RALY Mouse Oligoclonal Antibody #65291

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Flow cytometric analysis of RALY expression in HepG2 cells using anti-RALY antibody (Cat#65291, 1:2,000). Green, isotype control; red, RALY.

Immunocytochemical staining of HepG2 cells with anti-RALY antibody (Cat#65291, 1:1,000). Nuclei were stained blue with DAPI; RALY was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-RALY antibody (Cat#65291). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RALY antibody (Cat#65291, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-RALY antibody (Cat#65291). RALY expression in wild-type (WT) and RALY knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RALY antibody (Cat#65291, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

RALY
货号：

65291
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-RALY Mouse Oligoclonal Antibody
Aliases	RALY; RALY Heterogeneous Nuclear Ribonucleoprotein; HNRPCL2; P542; RNA-Binding Protein (Autoantigenic, HnRNP-Associated With Lethal Yellow); HnRNP Associated With Lethal Yellow Protein Homolog; Heterogeneous Nuclear Ribonucleoprotein C-Like 2; HnRNP Core Protein C-Like 2; RNA-Binding Protein Raly; Autoantigen P542; RNA Binding Protein, Autoantigenic (HnRNP-Associated With Lethal Yellow Homolog (Mouse)); RNA Binding Protein, Autoantigenic (HnRNP-Associated With Lethal Yellow Homolog); RNA-Binding Protein (Autoantigenic)
Background	Gene Name: RALY NCBI Gene Entry: 22913 UniProt Entry: Q9UKM9
Application Information	Molecular Weight: Predicted, 32 kDa; observed, 37 kDa Clonality: Mouse oligoclonal antibody Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human RALY
Isotype	Mouse IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of RALY expression in HepG2 cells using anti-RALY antibody (Cat#65291, 1:2,000). Green, isotype control; red, RALY.
Immunocytochemical staining of HepG2 cells with anti-RALY antibody (Cat#65291, 1:1,000). Nuclei were stained blue with DAPI; RALY was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RALY antibody (Cat#65291). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RALY antibody (Cat#65291, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-RALY antibody (Cat#65291). RALY expression in wild-type (WT) and RALY knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RALY antibody (Cat#65291, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

RALY
货号：

65291
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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