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Anti-SOX2 Rabbit Monoclonal Antibody #1826

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Flow cytometric analysis of SOX2 expression in HAP-1 cells using SOX2 antibody (Cat#1826, 1:2,000). Green, isotype control; red, SOX2.
Immunocytochemical staining of HAP-1 cells with SOX2 antibody (Cat#1826, 1:1,000). Nuclei were stained blue with DAPI; SOX2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SOX2 antibody (Cat#1826). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SOX2 antibody (Cat#1826, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    SOX2
  • 货号：
    1826
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-SOX2 Rabbit Monoclonal Antibody
Aliases SOX2; SRY-Box Transcription Factor 2; SRY (Sex Determining Region Y)-Box 2; Transcription Factor SOX-2; SRY-Box 2; Sex Determining Region Y-Box 2; SRY-Related HMG-Box Gene 2; Transcription Factor SOX2; MCOPS3; ANOP3
Background

Gene Name: SOX2

NCBI Gene Entry: 6657

UniProt Entry: P48431
Application Information

Molecular Weight: Predicted, 34 kDa; observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4645

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human SOX2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SOX2 expression in HAP-1 cells using SOX2 antibody (Cat#1826, 1:2,000). Green, isotype control; red, SOX2.
  • Immunocytochemical staining of HAP-1 cells with SOX2 antibody (Cat#1826, 1:1,000). Nuclei were stained blue with DAPI; SOX2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-SOX2 antibody (Cat#1826). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SOX2 antibody (Cat#1826, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    SOX2
  • 货号：
    1826
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of SOX2 expression in HAP-1 cells using SOX2 antibody (Cat#1826, 1:2,000). Green, isotype control; red, SOX2.
Immunocytochemical staining of HAP-1 cells with SOX2 antibody (Cat#1826, 1:1,000). Nuclei were stained blue with DAPI; SOX2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SOX2 antibody (Cat#1826). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SOX2 antibody (Cat#1826, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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