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Anti-YY1 transcription factor Rabbit Monoclonal Antibody #1882

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Flow cytometric analysis of YY1 transcription factor expression in HepG2 cells using YY1 transcription factor antibody (Cat#1882, 1:2,000). Green, isotype control; red, YY1 transcription factor.
Immunocytochemical staining of HepG2 cells with anti-YY1 transcription factor antibody (Cat#1882, 1:1,000). Nuclei were stained blue with DAPI; YY1 transcription factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-YY1 transcription factor antibody (Cat#1882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YY1 transcription factor antibody (Cat#1882, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    YY1
  • 货号:
    1882
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
50 μL,100 μL
价格:
¥ 600.00,¥ 1,000.00
Information
Product Name Anti-YY1 transcription factor Rabbit Monoclonal Antibody
Aliases YY1; YY1 Transcription Factor; INO80S; NF-E1; INO80 Complex Subunit S; YIN-YANG-1; DELTA; UCRBP; Transcriptional Repressor Protein YY1; Delta Transcription Factor; Yin And Yang 1 Protein; YY-1; Yin And Yang 1; GADEVS
Background

Gene Name: YY1

NCBI Gene Entry: 7528

UniProt Entry: P25490

Application Information

Molecular Weight: Predicted, 45 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4790

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human YY1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of YY1 transcription factor expression in HepG2 cells using YY1 transcription factor antibody (Cat#1882, 1:2,000). Green, isotype control; red, YY1 transcription factor.
  • Immunocytochemical staining of HepG2 cells with anti-YY1 transcription factor antibody (Cat#1882, 1:1,000). Nuclei were stained blue with DAPI; YY1 transcription factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-YY1 transcription factor antibody (Cat#1882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YY1 transcription factor antibody (Cat#1882, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of YY1 transcription factor expression in HepG2 cells using YY1 transcription factor antibody (Cat#1882, 1:2,000). Green, isotype control; red, YY1 transcription factor.
Immunocytochemical staining of HepG2 cells with anti-YY1 transcription factor antibody (Cat#1882, 1:1,000). Nuclei were stained blue with DAPI; YY1 transcription factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-YY1 transcription factor antibody (Cat#1882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YY1 transcription factor antibody (Cat#1882, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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