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Anti-CHRNA4 Rabbit Monoclonal Antibody #1911

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Flow cytometric analysis of CHRNA4 expression in HepG2 cells using CHRNA4 antibody (Cat#1911, 1:2,000). Green, isotype control; red, CHRNA4.

Immunocytochemical staining of HepG2 cells with anti-CHRNA4 antibody (Cat#1911, 1:1000) . Nuclei were stained blue with DAPI; CHRNA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-CHRNA4 antibody (Cat#1911). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHRNA4 antibody (Cat#1911, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CHRNA4
货号：

1911
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-CHRNA4 Rabbit Monoclonal Antibody
Aliases	CHRNA4; Cholinergic Receptor Nicotinic Alpha 4 Subunit; BFNC; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 4; Cholinergic Receptor, Nicotinic, Alpha 4 (Neuronal); Neuronal Acetylcholine Receptor Subunit Alpha-4; Cholinergic Receptor, Nicotinic Alpha 4; NACRA4; EBN1; EBN; Neuronal Nicotinic Acetylcholine Receptor Alpha-4 Subunit; Acetylcholine Receptor, Nicotinic, Alpha 4 (Neuronal); NACHRA4; NACHR
Background	Gene Name: CHRNA4 NCBI Gene Entry: 1137 UniProt Entry: P43681
Application Information	Molecular Weight: Predicted, 70 kDa; observed, 70 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5085 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human ACHA4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CHRNA4 expression in HepG2 cells using CHRNA4 antibody (Cat#1911, 1:2,000). Green, isotype control; red, CHRNA4.
Immunocytochemical staining of HepG2 cells with anti-CHRNA4 antibody (Cat#1911, 1:1000) . Nuclei were stained blue with DAPI; CHRNA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-CHRNA4 antibody (Cat#1911). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHRNA4 antibody (Cat#1911, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CHRNA4
货号：

1911
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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