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Anti-N-Myc And STAT Interactor Rabbit Monoclonal Antibody #2296

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Flow cytometric analysis of NMI expression in HT-1080 cells using anti-NMI antibody (Cat#2296, 1:2,000). Green, isotype control; red, NMI.
Immunocytochemical staining of HT-1080 cells with anti-NMI antibody (Cat#2296, 1:1,000). Nuclei were stained blue with DAPI; NMI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NMI antibody (Cat#2296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NMI antibody (Cat#2296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    NMI
  • 货号：
    2296
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-N-Myc And STAT Interactor Rabbit Monoclonal Antibody
Aliases N-Myc And STAT Interactor; N-Myc-Interactor; N-Myc (And STAT) Interactor; Nmi
Background

Gene Name: NMI

NCBI Gene Entry: 9111

UniProt Entry: Q13287
Application Information

Molecular Weight: Predicted, 35 kDa, observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB190

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human NMI
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:2,000, Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NMI expression in HT-1080 cells using anti-NMI antibody (Cat#2296, 1:2,000). Green, isotype control; red, NMI.
  • Immunocytochemical staining of HT-1080 cells with anti-NMI antibody (Cat#2296, 1:1,000). Nuclei were stained blue with DAPI; NMI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-NMI antibody (Cat#2296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NMI antibody (Cat#2296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    NMI
  • 货号：
    2296
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of NMI expression in HT-1080 cells using anti-NMI antibody (Cat#2296, 1:2,000). Green, isotype control; red, NMI.
Immunocytochemical staining of HT-1080 cells with anti-NMI antibody (Cat#2296, 1:1,000). Nuclei were stained blue with DAPI; NMI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NMI antibody (Cat#2296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NMI antibody (Cat#2296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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